This protocol details lipid analysis using MALDI mass spectrometry imaging in lung tissue, suggesting a new method for high-resolution lipidomics.
This protocol describes the procedure to obtain MALDI mass spectrometry images of lipids using a high mass resolution FTICR. This protocol is optimized for 12 µm sections of agarose-inflated and carboxymethyl cellulose-embedded fresh-frozen lung tissues. The embedding method is described in Lukowski et al. doi.org/10.3389/fmolb.2022.1022775
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Anderton et al. (2025) studied this question.
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