This protocol demonstrates simultaneous rna sequencing of multiple cardiac samples, highlighting transcriptomic variations.
By preserving cellular integrity at the point of collection, formalin-fixed paraffin-embedded (FFPE) captures fragile transcriptomic signatures across diverse cardiac cell types. This protocol supports multiplexed processing of fixed cells, enabling the concurrent single-cell RNA sequencing (scRNA-seq) of multiple heart tissue samples within a single experimental run. This advancement facilitates a deeper understanding of cardiac biology underlying heart disease at single-cell resolution, even in archived or clinical specimens.
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Vanegas et al. (2025) studied this question.
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