Gas Chromatography Mass Spectroscopy (GC-MS) Fingerprinting of Methanol Extract and Saponin-Rich Fractions of Corchorus olitorious (Jute) Leaves.

Gas Chromatography-Mass Spectrometry (GC-MS) is a powerful analytical technique used to identify and quantify bioactive compounds in complex mixtures like plant extracts. The study therefore, aimed at fingerprinting bioactive compounds of crude and saponin-rich fractions of Corchorus olitorius leaves using qualitative analysis and Gas Chromatography Mass Spectroscopy (GC-MS). Qualitative screening revealed moderate levels of phenols, tannins, flavonoids, glycosides, and alkaloids in the crude and most fractions, with saponins the most abundant in the ethanol (100%) fraction (++). The compounds with major peaks for GC-MS were: Fumaric acid, 2-methylpentyl 2,3-dichlorodichlorophenyl ester,4-Methyl-6-phenylpyrimidin-2-yl)(4,6,8-trimethylquinazolin-2-yl) amine, Fumaric acid, 4-heptyl tridecyl ester, and Dodecanoic acid, 1,2,3-propanetriyl ester in the crude extract. Ethanol 100 % fraction also revealed 4-Methoxy-3-nitrobenzyl alcohol, 4,4'-Bi-1,3-dioxolane, 2,2,2',2'-tetramethyl- and 2-(2-(2-Methoxyethoxy) ethoxy) ethyl pentanoate. While the Ethanol/methanol 80:20 fraction had Hexadecanoic acid methyl ester, Lauric anhydride, Citral, 2,6-Octadienal 3,7-dimethyl-, (Z)-. Ethanol/methanol 60:40 fraction further revealed Geranic acid, n-Hexadecanoic acid, Hexadecanoic acid, methyl ester, and 1,4-Cyclohexadiene, 6-isopropenyl-2,4-dimethyl-1,3-bis(trimethylsilyl)-. The crude and saponin-rich fractions of Corchorus olitorius leaves have revealed diverse classes of chemical compositions, which may be harnessed for therapeutic purposes.