Synthesis and Cytotoxicity Assessment of Curcumin-Derived Nickel Chloride Nanoparticles for ID8 mouse ovarian epithelial cancer cells

Background: In 2020, the US had approximately 1.8 million new cancer cases and over 606,000 cancer-related deaths. Between 1991 and 2017, overall cancer death rates decreased by 29%. This represents 2.9 million fewer cancer deaths than projected at high prevalence. Traditional tumor treatments like chemotherapy, radiation, and surgery are popular. Chemotherapy fights cancer but has major negative effects. Surgically excised tumors may reappear and resist radiotherapy. Aims: The objective of the current work was to evaluate the possible anticancer properties of nickel chloride nanoparticles synthesized from curcumin. Methods: 250 grams of curcumin powder and 500 ml of ethanol were combined. After mixing for an hour, we refrigerated the mixture at 4°C for 24 hours. The mixture was centrifuged for 10 minutes at 8,000 rpm. The supernatant was quickly collected and refrigerated for the next step. Next, we cooked 200 ML of the previous preparation on a 60 °C hotplate with 20 g of nickel chloride for 1 hour. Nano precipitates with NaOH. Next, we quickly centrifuged the mixture to extract nanoparticles. After two rinses with 5 ml of deionized water. Results: The image presents an X-ray Diffraction (XRD) spectrum utilized for identifying the crystalline structure and phase of a material—the pronounced and well-defined peaks in the spectrum, especially the most powerful peak at 2θ=43.310°. The NiO nanoparticles demonstrated the highest cytotoxic effects at 24 hours. Discussion: Elevated intracellular [Ni] levels result in decreased activity of Cu-dependent enzymes and transcription factors. Conclusions: Curcumin powder can be used to synthesize NiO nanospheres ecologically. Synthesized NiO nanoparticles are monoclinic and spherical, having a particle size of 20 nanometers. The synthesized nanoparticles are anticancer and have few adverse effects.