Protective effects of tomato extract gel on MMP-3 And IL-1 Beta levels in UVB-Induced skin inflammation in mice

Excess ROS triggers signal transduction and activates the nuclear factor kappa ? (NF-k?), an inflammatory mediator. The inflammatory response mediated by the heterodimers of the P50 and p65 NF-k? subunits induces the release of pro-inflammatory cytokines, one of which is interleukin-1? (IL-1?) which causes skin inflammation. Increased ROS also activates the protein kinase pathway which then triggers an increase in the production of the MMP-1 enzyme which then triggers an increase in MMP-3 secreted by neutrophils to degrade collagen. This study aims to determine the effect of giving tomato extract gel on MMP-3 and IL-1 levels in mice induced by acute UVB rays. UVB-induced skin damage involves inflammation and extracellular matrix degradation, yet the role of tomato extract gel as a topical antioxidant in mitigating these effects remains underexplored. This study investigates the potential of tomato extract gel in reducing MMP-3 and IL-1? levels through antioxidant and anti-inflammatory pathways. This study uses in vitro experiments with the Post Test Only Control Group Design method. The subject of this study was BALB/c mice divided into 4 treatments. The treatment consisted of negative control, positive control, administration of GEBT concentration of 10% and concentration of 20%. On the 7th day, MMP-3 and IL-1 levels were checked. The data was analyzed using the One Way Anova Test to determine the influence of each group, then continued with the Post Hoc LSD test to find out which dose had the most effect. The One Way Anova test showed the results of MMP-3 and IL-1 levels (there was a significant difference (p<0.05), the LSD Post Hoc Test showed significant differences in several comparison groups There was an effect of tomato extract gel administration on MMP-3 and IL-1 levels in mice exposed to acute UVB rays. Findings suggest that tomato extract gel could serve as an alternative therapeutic agent for managing UVB-induced skin damage.