In vitro establishment of cambuci [Campomanesia phaea (O. Berg) Landrum], a native fruit tree from the Brazilian Atlantic Forest

The cambuci [Campomanesia phaea (O. Berg) Landrum] is an endemic native fruit tree from the Brazilian Atlantic Forest. This study aimed to evaluate the in vitro response of nodal and leaf-derived explants collected from green house grown cambuci plants, including the explant in vitro establishment, and explant responses to culture media composition and to plant growth regulators. Nodal explants exhibited high contamination and oxidation rates, which can be reduced by collecting material during colder seasons. In vitro culture of nodalexplants with media supplemented with 2.0 mg L-1 of AgNO3 reduced the abscissionof new shoots, and the subculture of the material within 20-day intervals enhanced survival rates and decreased oxidation percentages. Cambuci leaf-derived explants treated with 0.75% sodium hypochlorite had low contamination and high oxidation rates, which, in turn, can be also reduced by supplementing of 1.0 g L-1 of polyvinylpyrrolidone(PVP). Leaf-derived explants exhibited no morphogenetic responses, while nodal explants had an increase in the number of new shoots with the supplementation of 11.1 µM BAP. The MS, 1/2 MS, 1/4 MS, WPM,1/2 WPM, JADS, and modified SPculture media were suitable for nodal explant cultivation. Subcultured cambuci shoots exhibited yellowing and necrosis, impacting survival rates.