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October 13, 2025Open Access

Protocol — DQ™ Red BSA Lysosomal Activity Assay HCS Analysis v1

Susana D. Lucas Giuseppe Uras Federico Fierli Sofia Koletsi David Chau Anthony H.V. Schapira

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Key Points

The protocol provides a functional readout for lysosomal proteolysis using DQ Red BSA, enhancing cellular analysis.
It employs confocal imaging and spot-based quantification methods to accurately analyze lysosomal activity.
This analysis allows researchers to visualize lysosomal function within cells, improving our understanding of cellular processes.
The implementation of this assay has broad implications for research in cellular dynamics and lysosomal diseases.

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Implication

Protocol assesses lysosomal proteolysis through confocal imaging, suggesting potential for cellular studies.

Abstract

Functional readout of lysosomal proteolysis using self-quenched DQ™ Red BSA, followed by confocal imaging and spot-based quantification.

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Cite This Study

Lucas et al. (2025) studied this question.

synapsesocial.com/papers/68f02c7d616531447b5f9404 https://doi.org/10.17504/protocols.io.rm7vz92ergx1/v1

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