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This is a Quantitative Study study.

October 16, 2025Open Access

Comparative Analysis of the Uterine Microbiome in Canine Pyometra: A 16S rRNA Sequencing Study

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This study compares the uterine microbiome in dogs with pyometra using 16S rRNA sequencing, revealing significant microbial dysbiosis.

Significant differences in microbial composition were found in pyometra-affected dogs compared to healthy controls.
Alpha diversity was markedly reduced in pyometra cases, confirmed by Shannon index results of 3.21 vs 1.89.
Specific families such as Pasteurellaceae and Lactobacillaceae were significantly enriched in pyometra cases.
Functional predictions indicated downregulation of key metabolic pathways associated with nutrient absorption and immune modulation.

Liao et al. (2025) studied this question.

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