What type of study is this?

This is a Experimental Study study.

October 17, 2025Open Access

HELIOS NAD-Seq: A Next-Generation Capture and Sequencing Protocol for NAD-Capped RNAs with Superior Targeting and Processing

View Full Paper

Ask AI

Bookmark

Authors

New protocol reduces RNA input for NAD-RNA sequencing in E. coli, highlighting improvements in specificity and throughput.

HELIOS NAD-Seq confirmed NAD-capping in 89.5% of transcripts and discovered 242 additional RNAs in E. coli.
The improved protocol achieves a reduction of at least 50-fold in RNA input requirements compared to existing methods.
Time-course profiling showed NAD-capping dynamics linked to metabolic state across different growth phases.
The method allows library preparation from 16 sample groups in quadruplicate within a four-day timeframe.

Möhler et al. (2025) studied this question.

View Full Paper

Ask AI

Bookmark