Optimization of fermentation conditions for enhancement of lipase production by Yarrowia isolates

Microbial lipases are widely used in biotechnology and industrial processes. Yarrowia lipolytica exhibits strong lipolytic activity and optimizing fermentation conditions is crucial for efficient enzyme production. This research investigated lipase production of some newly isolated Yarrowia strains, including Yarrowia lipolytica, Yarrowia divulgata , and Yarrowia yakushimensis . The Y. lipolytica 854/4 strain has reached the highest lipase activity after 3 days of fermentation, whereas other isolates require a longer fermentation time of 6–7 days. The lipase synthesis was not significantly affected by three tested cell count (10 6 , 5*10 6 and 10 7 CFU mL −1 ) during optimization of the initial inoculum concentration. Different concentrations of Triton X-100 and Tween 80 supplementation enhanced the lipase production, although the extent of this effect varied from strain to strain. These findings highlight Y. yakushimensis as a promising species, in addition to Y. lipolytica for industrial application involving lipase production.