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This is a Experimental Study study.

October 18, 2025Open Access

Long term cell culture of HeLa cells: Impact of Medium pH v1

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Protocol presents a method to stabilize medium pH during long-term HeLa cell culture, indicating its utility for chronic acidosis studies.

A stable acidic medium pH was maintained in HeLa cells over extended periods, enhancing culture reliability.
The method enables the study of chronic acidosis, with implications for developing pH-dependent therapies.
This approach provides a reproducible platform for future experimental designs targeting pH effects.
Careful management of medium pH is crucial for accurate evaluation of therapeutic interventions.

Tenorio-Salazar et al. (2025) studied this question.

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Protocol presents a method to stabilize medium pH during long-term HeLa cell culture, indicating its utility for chronic acidosis studies.