Synergistic Anti-Acne Activity of Bombax ceiba Thorn and Curcuma longa Gel in Testosterone-Induced Acne Vulgaris in Rodents

Abstract Purpose The present study purpose was to study and analyses the anti-acne activity of bombax ceiba thorn and curcuma longa combination against testosterone induced acne vulgaris on rodent. Methods Curcuma longa and Bombax ceiba thorn were gathered. to ascertain the bombax ceiba's Pharmacognostical assessment (phytochemical screening, microscopic estimation, physical evaluation, and macroscopic/organoleptic) to use GCMS analysis to assess Bombax cebia's phytoconstituents and in vitro antioxidant activities to create a gel-based formulation of bombax ceiba extracts with curcuma longa and assess the formulation's assessment parameters. To assess the anti-inflammatory and anti-acne properties of a gel-based formulation and a combination extract of bombax cebia and curcuma longa using an in vivo model of testosterone-induced acne to assess inflammatory markers like IL-1β and TNF-α. to assess, using histopathological analysis, the decrease in the number of sebaceous glands in the skin of mice. Result Treatment with EEBC + CL (**P < 0.05) the level of IL-1β TNF-α, MDA levels, oxidative stress, lipid peroxidation, reduction of sebaceous gland number, reduction of papules, inflammation reduced Conclusion The gel formulation with extracts of Bombax ceiba and Curcuma longa may be useful in treating testosterone-induced acne vulgaris, according to the study's findings. It improved antioxidant defence while dramatically lowering oxidative stress, TNF-α, IL-1β, and acne severity. Its safety and effectiveness need to be confirmed by additional clinical trials.