Caracterización de cepas de Pleurotus djamor obtenidas por cruzamiento dicarion-monocarion

Buller phenomenon consists of the dikaryoticization of monokaryotic mycelia bydikaryonic mycelia, was first observed in Coprinus lagopus and Schizophyllumcommune to determine incompatibility factors and understand the processes ofnuclear migration and clamp formation, but it has not been used as a geneticimprovement strategy in Pleurotus. The objective of the proposed work was toobtain and characterize di-monokaryonic strains of P. djamor native to Jalisco andtheir cultivation in tequila agave bagasse. To achieve this, the dikaryoticization ofmonosporic mycelia was studied by means of dikaryotic mycelia of three wild P.djamor strains from the state of Jalisco. A total of 9 di-monokaryonic strains wereobtained. Each strain obtained was evaluated for its performance in EMA and HITculture media, with the latter being the most suitable and therefore suitable for usein the management of these strains. Regarding agave carpophore production, itwas determined that they can reach up to 39% biological efficiency in fresh and4.9% in dry media. Furthermore, an average commercially suitable carpophoresize of 5 to 11 cm was established, resulting in percentages ranging from 77% to47% in the hybrid strains obtained.