Measurement of the Degree of Red Blood Cell Aggregation Based on the Automated Image Processing

A new method for measuring the quantitative indicator of the degree of red blood cell (RBC) aggregation in autologous blood plasma by automated analysis of images obtained using a light microscope is described. A method for measuring the degree of RBC aggregation in autologous blood plasma includes preprocessing, image acquisition and automated image analysis, image binarization, defining the boundaries of objects found, classifying objects into two classes (single RBC and aggregates) and calculating a numerical indicator reflecting the degree of RBC aggregation. The result of the treatment is an indicator of the degree of RBC aggregation in autologous blood plasma. The proposed method makes it possible to assess of RBC aggregation in autologous blood plasma and thereby obtain additional diagnostic and scientific information, increase the accuracy of assessing the degree of RBC aggregation in autologous blood plasma.