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Methodology identifies fish eggs at the species level, suggesting effective extraction and preservation techniques.
This DNA barcoding of fish eggs method is used to identify fish eggs down the the species level. Our lab has been using this method for about 10 years with good results and many publications. It is a destructive sampling method, meaning you will only have one chance to extract the DNA from the fish eggs. Once extracted, fish egg DNA samples have lasted in our -20 degree C freezers for over 5 years with good quality DNA. Many of the reagents used can be swapped for your preference.
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Breitbart et al. (2025) studied this question.
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