Biological Magnetic Iron Nanoparticles: A New Frontier in Ovarian Health Research

Magnetic iron oxide nanoparticles have attracted considerable attention in medical applications such as imaging and targeted drug delivery. In this study, biological magnetic iron nanoparticles were synthesized using Fusarium oxysporum and iron chloride. Their cytotoxicity was assessed using the MTT assay on ovarian cancer cells across ten stages. At stage 1, all treatment groups showed almost complete cancer cell death. To evaluate the magnetic responsiveness of the nanoparticles, cancer cells were exposed to a neodymium magnetic field in the presence and absence of nanoparticles, and intracellular iron concentration was measured using Inductively Coupled Plasma analysis. For in vivo assessment, 24 rats were divided into four groups and received intraperitoneal nanoparticle injections, with or without magnetic field exposure. The synthesized nanoparticles exhibited significant, dose‐dependent cytotoxicity. In later MTT assay stages, the nano‐drug group showed significantly lower cell viability compared to the free drug and nano‐carrier groups. Exposure to an electromagnetic field enhanced nanoparticle uptake into cells. Histological evaluation revealed no abnormal changes in healthy ovarian tissue. The biologically synthesized magnetic nanoparticles demonstrated effective, selective cytotoxicity against ovarian cancer cells, especially when combined with magnetic field exposure, while maintaining safety in healthy tissue.