This protocol demonstrates live-cell imaging of GCase activity in dopaminergic neurons, indicating vital insights into GBA1 activity monitoring.
This protocol describes the procedure for assessing GCase activity in iPSC-derived dopaminergic neurons using the Lyso-FQ GCase substrate (Biolegend 421948). The assessment is performed via live-cell imaging with the Opera Phenix high-content imaging system and analyzed using Harmony software. This LysoFQ-GBA (Lysosomal GCase Probe Green) probe, a cell-permeable reporter for GCase activity, fluoresces upon glucocerebrosidase activation, facilitating the detection and monitoring of GBA1 activity.
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Larivière et al. (2025) studied this question.