Evaluation of DNA Damage in Human Lymphocytes Exposed to Triclosan by Alkaline Comet Assay

In the present study, the in-vitro effect of Triclosan was tested on human peripheral lymphocytes taken in different concentrations. The genotoxicity of these antimicrobial agents was assessed using alkaline comet assay. The lymphocytes were isolated from the blood of non-smoker healthy students, which were treated with different concentrations of the selected antimicrobial agent i.e. Triclosan (2.5mg/mL, 4mg/mL, 5mg/mL, 6.5mg/mL, 7.5mg/Ml, 10mg/mL) and were kept for 1 hour in a dry bath incubator. The result demonstrated that Triclosan was able to induce DNA damage with all concentrations. The amount of damage was directly proportional to the concentration of antimicrobial drugs used.