Single-cell RNA sequencing reveals enhanced cellular communication and pro-inflammatory pathways in cervical lymph nodes infected with Mtb, indicating a complex immune response.
Key Points
The immune dynamics in cervical lymph nodes show extensive upregulation of pro-inflammatory pathways, highlighting the role of transcriptional reprogramming.
Single-cell RNA sequencing identified specific alterations in macrophages and T cells involved in cellular communication during Mtb infection.
Using computational network modeling and flow cytometry, a detailed analysis mapped immune cell heterogeneity across T, B, and myeloid subsets.
These findings provide valuable insights for developing therapies that target intercellular networks to balance immunity and pathological effects in tuberculosis.