Wpływ mikrocząstek i nanocząstek tlenku miedzi na wzrost grzybni Alternaria alternata, Botrytis cinerea i Fusarium oxysporum

The study aimed to compare the effect of CuxO micro- and nanoparticles (CuxO NPs) on the in vitro growth of selected plant pathogens: Alternaria alternata, Botrytis cinerea, and Fusarium oxysporum. In the in vitro experiment, pathogens were cultured on the PDA medium supplemented with CuxO or CuxO NPs at the concentration of 0, 100, 200, 500, 1000, or 2000 mg/l. Both CuxO and CuxO NPs significantly inhibited mycelial growth in all tested pathogens. In A. alternata, treatment with 500 mg/l of CuxO NPs was the most effective, reducing pathogen growth by an average of 76.64% compared to the control. A similar trend was observed in B. cinerea, where treatment with 500 mg/l CuxO NPs reduced pathogen growth by an average of 70.94%, indicating it as the most effective concentration. In contrast,F. oxysporum was only inhibited by 1000 and 2000 mg/l CuxO NPs or 2000 mg/l CuxO, which resulted in almost 100% growth inhibition. Although CuxO NPs showed promising antifungal properties, further study is necessary to evaluate their in vivo activity, environmental impact, plant tissue accumulation, and potential toxicity to non-target organisms. Understanding how these nanoparticles interact with the environment and their degradation pathways is vital for their safe application in agriculture.