Illumina sequencing reveals the chloroplast genome structure and morphology of Cladopus doianus, suggesting evolutionary insights for Podostemaceae.
Cladopus doianus Koriba 1928, a species within the Cladopus genus, possesses remarkable aerial shoots and an extensive underground root system, making it an exceptional model for investigating the evolutionary aspects of angiosperm morphology. In this study, we employed Illumina sequencing to assemble and annotate the complete chloroplast (cp) genome of C. doianus. The cp genome is 133,017 base pairs in length, containing 107 unique genes: 73 protein-coding genes, 30 tRNA genes, and four rRNA genes. Notably, 17 of these genes are duplicated within the inverted repeat (IR) regions, comprising four rRNA genes, seven tRNA genes, and six protein-coding genes. Additionally, we constructed a phylogenetic tree of selected species within the Podostemaceae family, and the results were consistent with previous studies, indicating that C. doianus is closely related to P. chiangmaiensis. In conclusion, our study presents the first complete chloroplast genome of C. doianus, providing valuable insights for future research on the Podostemaceae family.
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Yu et al. (2025) studied this question.
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