Global phosphoproteome analysis identifies PeVYV P4-induced host protein phosphorylation changes that facilitate viral proliferation

Our findings demonstrate that PeVYV P4-mediated phosphorylation modifications in N. tabacum are crucial for viral infection. This discovery of viral manipulation of host phosphorylation networks provides novel insights into the molecular mechanisms underlying PeVYV pathogenesis, and potential targets for developing PeVYV-resistant cultivars through molecular breeding approaches. These results significantly advance our understanding of the evolutionary arms race between plants and PeVYV at the post-translational modification level.