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November 19, 2025Microbial GenomicsOpen Access

Low-volume Plasmodium blood sample processing protocols for untargeted transcriptomics optimized using Plasmodium knowlesi

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Authors

ESErin SauveJKJohanna Helena KattenbergPMPieter Moris

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Overview

This research demonstrates enhanced transcriptomic outcomes through white blood cell depletion in low-parasite samples, indicating a pathway for Plasmodium analysis.

Key Points

  • This research aims to optimize sample processing protocols for untargeted transcriptomics in Plasmodium species.
  • Utilized Plasmodium knowlesi culture diluted in whole blood as a model for P. vivax infection.
  • Compared various white blood cell (WBC), rRNA, and globin depletion techniques.
  • Evaluated RNA-seq library preparation kits to find the most effective combination for low-volume processing.
  • WBC depletion increased the percentage of mapped Plasmodium paired-end reads from 5.4% to 37.2-54.5%.
  • Globin and rRNA depletion methods decreased human globin RNA and rRNA counts by up to 90%.
  • mRNA library preparation kits showed high correlation and enrichment for protein-coding genes.

Cite This Study

Sauve et al. (2025) studied this question.

synapsesocial.com/papers/6924fed8c0ce034ddc351252https://doi.org/10.1099/mgen.0.001546
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