CRSwNP Research: Insights into Pathogenesis, RBP5 and GDF15 - related Discoveries 9134

Description Method Proteomics was used to identify protein expression in polyps or inferior turbinates of CRSwNP, CRSsNP patients and normal controls. Flow cytometry and immunofluorescence located protein expression, while PCR, WB, ELISA and immunohistochemistry were applied for quantitative analysis. siRNA was constructed to knockdown genes, and plasmids were used for over - expression. Result Proteomics revealed a decrease in RBP5 in the CRSwNP group. Flow cytometry and immunofluorescence showed that RBP5 was mainly located in the nasal mucosal epithelium, with its expression in the nasal mucosal epithelium of the CRSwNP group lower than that in the CRSsNP group and healthy controls. After knocking down RBP5, tPA and atRA in the supernatant and cell lysate were significantly down - regulated, and there was a positive correlation among the three. RBP5 was down - regulated after Poly(I:C) stimulation, and this down - regulation disappeared after knocking down TLR3. Transcriptome sequencing after over - expressing RBP5 found an up - regulation of GDF15. Adding recombinant human GDF15 to epithelial cells could inhibit the activation of NLPR3 and the production of IL - 1β. Conclusion This study provides new insights into the pathogenesis of CRSwNP. RBP5 is expected to be a potential therapeutic target for CRSwNP, and the discovery of GDF15 offers new ideas for treating nasal inflammation. Future research may focus on RBP5 and GDF15 to explore innovative treatment strategies for CRSwNP.