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This research aims to profile tumor-infiltrating lymphocytes in soft tissue sarcomas to evaluate their characteristics and clinical significance.

November 25, 2025The Journal of ImmunologyOpen Access

Immune profiling of tumor-infiltrating lymphocytes in patients with primary and metastatic soft tissue sarcomas 3352

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Analysis shows immune checkpoint inhibitors influence tumor-infiltrating lymphocyte profiles in sarcomas, indicating impacts on treatment response.

This research aims to profile tumor-infiltrating lymphocytes in soft tissue sarcomas to evaluate their characteristics and clinical significance.
Analyzed TILs and PBMCs from sarcoma patients using multiplex flow cytometry.
Compared phenotypes and states between matched TILs and PBMCs using FlowJo and Graphpad Prism.
Utilized unpaired Mann-Whitney tests for statistical analysis.
TILs exhibited a higher CD8:CD4 ratio than PBMCs (median ratio TILs 1.05 vs PBMCs 0.25).
Detected a higher proportion of CD8+CD103+CD39+ T cells in TILs compared to PBMCs (4.3% vs 0.09%).
Most TILs were found to have an effector memory phenotype (median %CD8+EM+ 61.2 and %CD4+EM+ 81.6).
Higher expression of PD1 was observed in TILs compared to PBMCs (mean MFI 7057 vs 1924).

Chaudhry et al. (2025) studied this question.

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Analysis shows immune checkpoint inhibitors influence tumor-infiltrating lymphocyte profiles in sarcomas, indicating impacts on treatment response.