Development of laboratory modules in virology suitable for the undergraduate classroom 3278

Description Decades of virology research has led to the development of vaccines and antivirals against common pathogens, their potential to alter genomes, novel approaches to drug delivery, and even the ability of some viruses to specifically destroy cancer cells. However, the increasing rates of vaccine hesitancy, rampant misinformation, and distrust of science have resulted in a negative public perception towards virology research. Although this problem could be logically ameliorated through education, most undergraduate students do not have access to a stand-alone virology course. In here, we present the development of a series of undergraduate-friendly laboratory modules that can be implemented in college classroom. We take advantage of Orthoreovirus, a simple, fast-replicating, and genetically tractable RNA virus that infects many types of human cell lines. This virus imposes no harm to healthy individuals and thus is commonly used under Biosafety Level 2 (BSL-2) regulations as a surrogate for its pathogenic counterparts. Working with undergraduate trainees, we have developed modules that exemplify key virology principles such as viral entry, innate immune responses, antibody-mediated neutralization of viruses, tracking genetic drift and shift, and oncolytic virotherapy—all recommended learning objectives proposed by the American Society for Microbiology. Altogether, our laboratory modules provide a framework for the refinement of undergraduate laboratory activities in virology. Funding Sources Supported by Elon University Topic Categories Immunology Education and Communication (EDU)