Murine gammaherpesvirus68 drives demyelination in a novel model of MS, cuprizone autoimmune encephalomyelitis which may better represent disease initiation 2065

Description Multiple sclerosis (MS) is an autoimmune disease and among the leading causes of disability in young adults. A primary animal model for studying MS, experimental autoimmune encephalomyelitis (EAE), relies on exogenous antigen and induces severe ascending paralysis in a number days. EAE represents an “outside in” disease model. Since the exogenous antigen is delivered peripherally, cells active against antigen migrate into the CNS and induce primary myelin loss. Increasing data suggest that MS displays qualities of an “inside out” model of diease where primary loss of oligodendrocytes precedes demyelination. Previous research in our lab showed Th1 skewing and more severe EAE in mice with latent murine gamma herpesvirus 68 (gHV68) infection. gHV68 is a murine homologue of EBV. EBV is strongly associated with MS pathology (Bjornevik et al. 2022) Curpizone autoimmune encephalomyelitis (CAE) (Caprariello et al. 2018), is another model of MS. In CAE, cuprizone chow is fed to mice to induce oligodendrocyte death, followed by an immune boost, resulting in inflammatory demyelination. Cuprizone leads to accumulated citrullinated myelin which is also overrepresented in MS (maybe linked to smoking). CAE represents an “inside out” disease model. Our lab found that latent gHV68 also increases demyelination in CAE and skews immune response toward a TH1 phenotype. CAE with gHV68 may provide a more accurate MS model by mimicking environmental triggers and better representing disease initiation Funding Sources Andrew Nord fellowship, University of British Columbia Topic Categories Basic Autoimmunity (BA)