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November 13, 2025Microbiology SpectrumOpen Access

Oxidoreductase gene fabG contributes to fungal development, cell wall integrity, and virulence in Aspergillus fumigatus

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Authors

HZHeng ZhangHNHua NiXTXinyi Tao

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Overview

Knockout of fabG reduced virulence and antifungal susceptibility in Aspergillus fumigatus, indicating redox balance's role in its physiology.

Key Points

  • This research explores the role of the oxidoreductase gene fabG in Aspergillus fumigatus growth and virulence.
  • fabG knockout (Δ fabG) strains were created through homologous recombination.
  • Phenotypic assays assessed hyphal growth, virulence, and antifungal susceptibility.
  • Transcriptomic profiling and cell wall composition analyses were performed to evaluate redox homeostasis and cell wall integrity.
  • Macrophage co-culture experiments were conducted to analyze phagocytosis and macrophage killing.
  • Δ fabG exhibited inhibited hyphal growth and reduced virulence against Galleria mellonella.
  • Transcriptomics revealed changes in oxidoreductase and MAPK signaling genes.
  • There was increased chitin and β-glucan in the Δ fabG strain, indicating cell wall thickening.
  • Δ fabG showed decreased SOD activity and elevated ROS levels, reducing stress tolerance.

Cite This Study

Zhang et al. (2025) studied this question.

synapsesocial.com/papers/692523bbc0ce034ddc354a10https://doi.org/10.1128/spectrum.02092-25
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