Enzymatic Synthesis of Structured Phospholipids Containing Pentadecanoic Acid: A Novel Method for Industrial Applications

Structured phospholipids (SPLs) enriched with pentadecanoic acid (C15:0, PDA) were synthesized via enzymatic acidolysis. Among several commercial preparations, a new immobilized product from the Rhizomucor miehei lipase was identified as the most effective catalyst in the hexane system. A single‐factor study was conducted to establish foundational reaction conditions, which guided a subsequent optimization. A quadratic model was developed to evaluate the interactions of substrate molar ratio, enzyme load, and reaction time. The optimal conditions for laboratory scale production were obtained with a molar ratio of PDA/PL (8.44:1), an enzyme load of 18.5%, and a reaction time of 14.46 h. Production under these conditions incorporates PDA up to 85.8%. The study demonstrates a much‐improved reaction system to produce SPLs containing PDA.