What question did this study set out to answer?

To develop a high-throughput droplet-based protocol for profiling RNA and chromatin accessibility in single cells.

November 7, 2025Blood ScienceOpen Access

A drop-based workflow for fast and robust joint profiling of transcriptome and chromatin accessibility in the same cell

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Authors

ZYZiying Yang SLSiqi Liu CCChangya Chen

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Implication

This protocol enables simultaneous RNA and chromatin profiling in single cells, suggesting enhanced gene expression analysis and parallel processing capabilities.

Key Points

To develop a high-throughput droplet-based protocol for profiling RNA and chromatin accessibility in single cells.
Utilized droplet-based microfluidics for profiling
Performed cell pretreatment and nuclei isolation
Generated gel bead-in-emulsion (GEM) for scRNA-seq and scATAC-seq libraries
Supported parallel processing of thousands of cells
Enhanced sensitivity and specificity in identifying rare cell populations
Allowed for integrative analysis of gene expression and chromatin accessibility
Achieved exceptional scalability and reproducibility in experiments

Cite This Study

Yang et al. (2025) studied this question.

synapsesocial.com/papers/69253a39c0ce034ddc357d03 https://doi.org/10.1097/bs9.0000000000000258

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Implication

This protocol enables simultaneous RNA and chromatin profiling in single cells, suggesting enhanced gene expression analysis and parallel processing capabilities.

A drop-based workflow for fast and robust joint profiling of transcriptome and chromatin accessibility in the same cell

Discussion

Member takes

Implication

Key Points

Cite This Study

A drop-based workflow for fast and robust joint profiling of transcriptome and chromatin accessibility in the same cell

Discussion

Member takes

Implication

Key Points

Cite This Study

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