Limiting the effects of radiation damage in MicroED through dose selection during data processing

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Analysis shows reduced radiation damage and enhanced structure determination in MicroED, highlighting efficient data-processing strategies.

Radiation damage affects macromolecular crystal structures, compromising structural integrity and analysis accuracy.
Macromolecular crystal structures were studied to enhance structure determination across multiple data sets.
Data-processing strategies were employed to mitigate radiation damage during electron diffraction data collection.
Improving structure determination can lead to more accurate and reliable structural biology outcomes.

Bwanika et al. (2025) studied this question.

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Analysis shows reduced radiation damage and enhanced structure determination in MicroED, highlighting efficient data-processing strategies.