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October 25, 2025Caraka Tani Journal of Sustainable AgricultureOpen Access

Optimization of Plant Growth Regulators and Bioreactor Systems for Efficient In Vitro Shoot Multiplication and Elongation of Amorphophallus muelleri Blume

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Authors

NJNur Afni JayantiAPAngelia Krisdanti Nasera PutriYMYosephine Sri Wulan Manuhara

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Overview

This research demonstrates improved shoot multiplication in Amorphophallus muelleri using plant growth regulators and bioreactor systems, indicating potential for large-scale propagation.

Key Points

  • To optimize plant growth regulators and bioreactor systems for effective in vitro shoot multiplication and elongation of Amorphophallus muelleri.
  • Implemented combinations of thidiazuron and benzylaminopurine or kinetin on MS media for shoot induction.
  • Measured the effects of different combinations on shoot induction and shoot number over 8 weeks.
  • Cultured induced shoots in Temporary Immersion System and Balloon Type Bubble Bioreactor for elongation assessment.
  • TDZ 0.50 mg l⁻¹ + BAP 1.00 mg l⁻¹ yielded the fastest shoot induction (32.34 days).
  • TDZ 0.75 mg l⁻¹ + BAP 0.75 mg l⁻¹ resulted in 76.33 shoots per explant with 100% shoot formation.
  • The Balloon Type Bubble Bioreactor produced a significantly higher shoot output compared to Temporary Immersion System.

Cite This Study

Jayanti et al. (2025) studied this question.

synapsesocial.com/papers/69254f92c0ce034ddc359ba2https://doi.org/10.20961/carakatani.v40i4.103957
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The Effect of Benzyl Amino Purin and NaphtalenaAcetic Acid Applications on Direct Shoot Organogenesis in Porang (Amorphophallus muelleri B)2024 · 1 citations
  2. 2Response of Shoots from Porang Leaf Bulbs to Cytokinins and IAA in Shoot Multiplication In Vitro2024
  3. 3PEMANFAATAN BAP DENGAN VARIASI KONSENTRASI DAN LAMA PERENDAMAN UNTUK MERANGSANG PERTUMBUHAN TUNAS BULBIL PORANG (Amorphophallus muelleri Blume)2025
  4. 4Effect of Light Color and Auxin on Callus Induction and Development in <i>Amorphophallus muelleri</i> Blume.2025
  5. 5Establishment and Shoot Development Responses of Hylocereus undatus Under Plant Growth Regulator Treatments2026