Accurate estimation of individual tree aboveground biomass (AGB) is essential for understanding forest carbon dynamics, optimizing resource management, and addressing climate change. Conventional methods rely on destructive sampling, whereas unmanned aerial vehicle (UAV) remote sensing provides a non-destructive alternative. In this study, spectral indices, textural features, and canopy height attributes were extracted from high-resolution UAV optical imagery and Light Detection And Ranging (LiDAR) point clouds. We developed an improved YOLOv8 model (NB-YOLOv8), incorporating Neural Architecture Manipulation (NAM) attention and a Bidirectional Feature Pyramid Network (BiFPN), for individual tree detection. Combined with a random forest algorithm, this hybrid framework enabled accurate biomass estimation of Chinese fir, Chinese pine, and larch plantations. NB-YOLOv8 achieved superior detection performance, with 92.3% precision and 90.6% recall, outperforming the original YOLOv8 by 4.8% and 4.2%, and the watershed algorithm by 12.4% and 11.7%, respectively. The integrated model produced reliable tree-level AGB predictions (R2 = 0.65–0.76). SHapley Additive exPlanation (SHAP) analysis further revealed that local feature contributions often diverged from global rankings, underscoring the importance of interpretable modeling. These results demonstrate the effectiveness of combining deep learning and machine learning for tree-level AGB estimation, and highlight the potential of multi-source UAV remote sensing to support large-scale, fine-resolution forest carbon monitoring and management.
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