Development and Validation of the Method for the Quantification of Hydroxycinnamic Acids in (Lamiaceae) Herb

Korean mint or wrinkled giant hyssop ( L.) is one of the promising plant sources for the production of medicinal herbal preparations. In this work a technique that has been developed for the quantitative determination of the amount of hydroxycinnamic acids in the herb of by direct spectrophotometry is described. The analytical maxima of the studied compounds are 330 nm and the shoulder is 290 nm. The optimal conditions for the extraction of hydroxycinnamic acids from raw materials are substantiated. Chlorogenic acid was chosen as a reference sample for calculating the content of hydroxycinnamic acids. The developed method is evaluated according to the validation characteristics: specificity, linearity, precision, correctness and is found suitable for use in analytical purposes. It was revealed that the content of hydroxycinnamic acids in the herb of is 7.50% of the weight of absolute dry raw materials.