Rapid, Reliable, and Green: A Novel RP‐HPLC Method for Quercetin Estimation in Green‐Synthesized Nanoparticles

Quercetin (QUE) is a natural flavonoid phyto‐constituent found in fruits and vegetables with diverse medicinal properties. Green synthesis nanoparticles using plant extracts have gained attention for their eco‐friendly and safe approach. The present work aimed to develop a facile, accurate, and reliable reverse‐phase high‐performance liquid chromatography (RP‐HPLC) technique to determine QUE in nanoparticles. Chromatographic separation was done with a C18 column (4.6 × 250 mm, 5 μm) with a mobile phase of methanol and 0.1% orthophosphoric acid (OPA) (80:20, v/v) with a flow rate of 1.0 mL/min at 371 nm. The method developed for QUE proved to be highly linear in the range of 0.1–8.0 μg/mL ( R 2 >0.999) with a retention time of 3.5 min. The limit of detection (LOD) and limit of quantification (LOQ) were 0.6 and 2.1 μg/mL, respectively. Validation parameters such as accuracy, precision, robustness, and specificity were in accordance with ICH guidelines. The novelty of the research work depends on attaining a maximum of QUE at a lower retention time compared to conventional methods.