Phytochemical screening and antibacterial activity of green synthesis of nanoparticles of Punica granatum

The main objective of this work was to explore Punica granatum phytochemicaly and synthesize nanoparticles from its polar extract. The phytochemical screening test of ethanolic and aqueous extract of P. granatum indicated the presence of tannins, flavonoides, emodins, terpenoids, cardiac glycosides, coumarine, and soluble starch. The aqueous extract showed the presence of tannins, flavonoides, terpenoids, emodins, cardiac glycosides, caumarine, carbohydrates and soluble starch. The extracts were subjected to synthesize gold nanoparticles (Au-NPs) Synthesized Au-NPs were characterized by using Uv-visible, Fourier transform (FT-IR) spectroscopy and AFM (atomic force microscope) analysis. The active phytochemical present in extract are responsible for synthesizes gold nanoparticles (Au-NPs). In addition, synthesized Au-NPs and the methanolic extracts of Punica granatum were screened for their in-vitro antioxidant activity. Au-NPs showed excellent antioxidant potentials. It is concluded that Punica granatum extracts is an outstanding bioreducant for the rapid and green synthesis of Au-NPs, which in turn showed various antioxidant activity.