December 6, 2025PLoS ONEOpen Access

Comparison between Legiolert and real time PCR in the detection of Legionella pneumophila from environmental water samples

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Assessment compares detection performance of Legiolert and real-time PCR for Legionella pneumophila, suggesting the screening tool aids water safety.

Real-time PCR demonstrated a significant performance gap compared to the gold standard culture method for detecting Legionella pneumophila in water.
McNemar test results indicated that the culture method is more accurate than real-time PCR, emphasizing its role as the current gold standard.
Analysis conducted on 75 environmental water samples, tested in parallel with Legiolert and real-time PCR for comprehensive assessment.
Legiolert offers a potential screening tool for rapid detection, but the study highlights the need for standardized procedures in quantification.

Arrigo et al. (2025) studied this question.

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Assessment compares detection performance of Legiolert and real-time PCR for Legionella pneumophila, suggesting the screening tool aids water safety.