DNA barcode analyses and taxonomy reveal two new species of Inocybe from Pine and Oak forests of Pakistan

Here we describe two new species of Inocybe from pine forests of Pakistan; I. hazarensis and I. shimlaensis . Morphological and molecular data show that these species have not been described before and hence need to be described as new. Both species are smooth‐spored and pruinose only in the apical part of the stipe. Inocybe hazarensis is characterized by rather small size, brown to dark brown, dense radial fibrils to rimose to glabrous pileus with prominent umbo, finely fibrillose at apex only becoming pruinose to strigose, along rest of length of stipe, slightly bulbous stipe base, narrow basidiospore spores and smaller clavate, oblong, ovoid, narrowly utriform cheilocystidia. Inocybe shimlaensis is characterized by brown to golden pileus, low and broad umbo, radially fibrillose, rimose to granulose, with brown appressed‐squamulose stipe with only apex pruinose and submarginate base. Anatomically, it has small (6.8 × 4.4 μm) basidiospores and smaller conical to fusiform cheilocystidia. Phylogenetic estimation based on DNA sequences from the internal transcribed spacer (ITS) region and large subunit (LSU) of the nuclear ribosomal DNA (rDNA) genes is congruent with the morphological characters that help to delimit these as new species of Inocybe .