What question did this study set out to answer?

To understand how RUNX1 functions as a transcription factor in normal and leukemic cells.

December 8, 2025Blood

Context-dependent recruitment of co-factors directs RUNX1 activity in normal and leukemic cells

View Full Paper

Ask AI

Bookmark

Authors

Transcription factor RUNX1 alters gene regulation in acute myeloid leukemia, suggesting its dual role in activation and repression.

To understand how RUNX1 functions as a transcription factor in normal and leukemic cells.
Used in vivo tethering system to study RUNX1 effects on chromatin context.
Performed ChIP-seq analysis in RUNX1-dependent ALL cell line SEM.
Investigated RUNX1 knockdown in KMT2A::AFF1 cells and overexpression in mouse embryonic stem cells.
RUNX1 recruits both co-activators and co-repressors.
Positive correlation found between RUNX1 binding and active histone modifications like H3K4me3.
C-terminally truncated RUNX1 mutant fails to recruit Polycomb PRC1 complex.

Dimou et al. (2025) studied this question.

View Full Paper

Ask AI

Bookmark