Single-cell analysis reveals mutant cell effects on stem cells in clonal hematopoiesis, indicating altered immune signaling.
Key Points
This research investigates the role of bone marrow environmental changes in clonal hematopoiesis. It focuses on the effects of somatic mutations on hematopoietic stem cells.
Established a single-cell multi-omics platform for analysis of hematopoietic stem and progenitor cells.
Analyzed Lineage⁻ CD34⁺ HSPCs from individuals with and without clonal hematopoiesis.
Conducted competitive BM transplantation assays with mutant and wild-type cells in mice.
Identified consistent gene expression upregulation in mutant HSPCs compared to wild-type cells.
Mutant cells showed diminished responses to proinflammatory cytokines relative to controls.
Found that bone marrow environment is altered in clonal hematopoiesis, affecting immune cell composition.