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December 8, 2025BloodOpen Access

TADA1 plays a regulatory role in erythropoiesis and the expression of HBG.

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Authors

DHDawei HuoRWRui WenHHHe Huang

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Overview

Genome-wide screen identifies TADA1's impact on hemoglobin regulation, suggesting new therapies for anemia.

Key Points

  • This research investigates TADA1's regulatory influence on erythropoiesis and HBG expression.
  • Performed a genome-wide CRISPR-Cas9 screen to identify regulatory roles of TADA1.
  • Validated findings in HUDEP2 cells and primary CD34+ hematopoietic stem cells using Western blotting and qPCR.
  • Analyzed adult bone marrow single-cell transcriptomic data to assess TADA1 expression in erythroid progenitors.
  • Conducted targeted screening of the SAGA complex subunits for their effect on HBG induction.
  • TADA1 knockout significantly increased the proportion of HbF-positive cells.
  • Identified TADA1 as a major regulator of γ-globin expression in erythroid differentiation.
  • Discovery that TADA1 mediates HBG expression regulation through dual mechanisms involving transcription factors and ribosomal proteins.

Cite This Study

Huo et al. (2025) studied this question.

synapsesocial.com/papers/69362f364fa91c937236d2f8https://doi.org/10.1182/blood-2025-730
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