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December 8, 2025BloodOpen Access

A human IL-6–Expressing PDX model of multiple myeloma recapitulates clinical features and supports patient-derived tumor engraftment

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Authors

YZYan ZengVSVeronica SteriESElliot Stieglitz

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Overview

PDX model showcases bone disease and therapeutic targets in multiple myeloma, indicating potential for drug testing.

Key Points

  • To develop a human IL-6–expressing PDX model reproducing multiple myeloma clinical features.
  • Developed NOG mice expressing human IL-6 and engrafted with primary MM cells.
  • Conducted serum IL-6 quantification using immunoassays and monitored tumor engraftment via serum protein electrophoresis.
  • Performed flow cytometry to analyze bone marrow plasma cell infiltration and evaluated hematologic parameters.
  • Four of six mice developed a monoclonal gamma spike as detected by serum protein electrophoresis.
  • Confirmed anemia in engrafted mice compared to controls, evidenced by blood counts.
  • Detected bone disease characteristics via imaging and flow cytometry analysis.

Cite This Study

Zeng et al. (2025) studied this question.

synapsesocial.com/papers/69362f3a4fa91c937236d3a8https://doi.org/10.1182/blood-2025-3943
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Human IL-6 fosters long-term engraftment of patient derived disease-driving myeloma cells in immunodeficient mice2024 · 9 citations
  2. 2In Vivo postnatal loss of mature osteoblasts creates an immunosuppressive bone marrow microenvironment to permit the engraftment and proliferation of myeloma cells2025
  3. 3Abstract 3385: Humanized mouse models 2.0 - Improved preclinical evaluation of novel immune cell therapies, check point inhibitors, and immune cell engagers by excluding false positive or negative results2026
  4. 4Abstract 3390: Enhanced human immune system engraftment in a novel triple-engineered NCG-hIL6-mTSLP-mFlt3-KOmouse model2026
  5. 5Abstract 4215: Single-cell proteomic characterization of the tumor microenvironment in humanized NOG-EXL patient-derived xenograft (PDX) models2024