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December 8, 2025BloodOpen Access

The m5C-binding protein YB-1 modulates proteasome inhibitors resistance in multiple myeloma via epigenetic modification

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Authors

ZCZhen Cai

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Overview

In vitro findings demonstrate YB-1's role in drug resistance in multiple myeloma, indicating potential therapeutic targets to enhance chemoresistance management.

Key Points

  • This research aims to elucidate the role of YB-1 in proteasome inhibitors resistance in multiple myeloma through RNA 5-methylcytosine modification.
  • In vitro experiments assessing YB-1 knockdown effects on MM cells
  • Xenograft models using MM cell lines with YB-1 knockdown in NCG mice
  • High-throughput sequencing analyses including m5C-Bis-seq, RIP-seq, and RNA-seq
  • YB-1 knockdown induced increased apoptosis and G2 phase arrest in MM cells under proteasome inhibitors treatment
  • YB-1 KD significantly reduced tumor burden and improved survival in xenograft models
  • Restoration of YB-1 expression rescued MM cell survival under proteasome inhibitors treatment, highlighting its role in drug resistance

Cite This Study

Zhen Cai (2025) studied this question.

synapsesocial.com/papers/69362f3a4fa91c937236d3ebhttps://doi.org/10.1182/blood-2025-5700
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