Chromodomain helicase DNA binding protein CHD2 interacts with TET2 and regulates lineage defining transcription in hematopoietic stem and progenitor cells
Research shows CHD2 regulates gene expression and differentiation in hematopoietic stem cells, implicating its role in myeloid malignancies and epigenetic processes.
Key Points
This research investigates the interaction between CHD2 and TET2 and its effects on hematopoietic stem cells.
Utilized CRISPR-Cas9 to create TET2 knockout (TET2KO) and wild-type (TET2WT) cells from K562 and THP1.
Performed immunoprecipitation to analyze the CHD2-TET2 interactome using mass spectrometry.
Conducted integrative multi-omics analysis with proteomics, ChIP-seq, and RNA-seq to evaluate the impact of TET2 loss on gene expression.
CHD2 and TET2 exhibit a robust interaction, with significant overlap found in their interactomes.
Differential expression of genes was noted in TET2KO cells, with oncogenic pathways being upregulated.
The majority of CHD2 and TET2 target genes showed 95% overlap, highlighting their cooperative regulatory role.