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December 8, 2025Blood

Lattice light-sheet microscopy reveals patient specific responses to CD20-directed antibodies in B cell malignancies

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Authors

PEPatrick EiringSHSeungbin HanMSMax Steinhardt

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Overview

Lattice light-sheet microscopy reveals unique binding patterns and cytoskeletal responses to CD20 antibodies in hematologic malignancies, suggesting implications for personalized therapies.

Key Points

  • The study aims to explore the real-time responses of B cells to CD20-directed antibodies using lattice light-sheet microscopy.
  • Used lattice light-sheet microscopy to visualize interactions of mAbs with B cells.
  • Applied fluorescently labeled mAbs and C1q to Raji cells and primary B cells from CLL patients.
  • Observed cytoskeletal changes using SiR actin to measure membrane protrusions and polarization during imaging.
  • Ofatumumab showed reduced C1q complex binding compared to rituximab.
  • C1q binding varied significantly between different CLL patient samples.
  • Cytoskeletal changes were observed with varying degrees of polarization among treatments.

Cite This Study

Eiring et al. (2025) studied this question.

synapsesocial.com/papers/69362f484fa91c937236d670https://doi.org/10.1182/blood-2025-4343
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Cytotoxic CD8⁺ T cells are required for CD20xCD3 bispecific antibody-mediated tumor control in B-cell non-Hodgkin lymphomas2025
  2. 2Abstract PR06: Comprehensive characterization of the non-tumor microenvironment of relapsed/refractory large B-cell lymphoma identifies patients with greatest benefit from CD19 CAR T-cell therapy2024
  3. 3Abstract 4357: Novel monoclonal antibody kills lymphoma cells via LFA-1/ICAM-1 dependent, antibody-mediated cellular cytotoxicity2026
  4. 4Genomic and immune determinants of response to CD3 x CD20 bispecific antibodies in aggressive B-cell lymphoma2025
  5. 5Foreign antigen delivered to CLL B cells via membrane IgD leads to a distinct antigen processing pathway that allows antigen-specific T cell activation that is superior to membrane IgM delivery2025