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December 8, 2025BloodOpen Access

Beyond hemostasis: Factor VIII deficiency impacts  hematopoietic stem cells development in zebrafish

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Authors

WAWatfa AhmedZAZainab AwadaGGGiusy Gentilcore

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Overview

Knockdown of Factor VIII disrupts hematopoiesis in zebrafish, suggesting altered stem cell mobilization and metabolism.

Key Points

  • This research aims to explore the impact of Factor VIII deficiency on hematopoiesis and stem cell development in zebrafish.
  • Used morpholino injections to knock down Factor VIII in zebrafish models.
  • Conducted RNA-seq analyses and flow cytometry sorting at different maturation stages.
  • Performed colony-forming unit assays with CD34+ hematopoietic cells exposed to recombinant Factor VIII.
  • Identified significant transcriptomic changes in hematopoietic lineages due to Factor VIII knockdown.
  • Observed impaired myeloid-erythropoiesis and increased myeloid output in f8-KD zebrafish.
  • Demonstrated a linear increase in CFU-GM colonies when CD34+ cells were exposed to Factor VIII.

Cite This Study

Ahmed et al. (2025) studied this question.

synapsesocial.com/papers/69362f714fa91c937236e230https://doi.org/10.1182/blood-2025-4933
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Loss of factor VIII in zebrafish rebalances antithrombin deficiency but has a limited bleeding diathesis2024 · 1 citations
  2. 2Osteoblasts differentiated from FVIII-deficient mice exhibit impaired mineralization capacity and promote excessive in vitro osteoclastogenesis2025
  3. 3Factor VIII originates primarily from anatomically distinct subsets of liver sinusoidal endothelial cells2026 · 1 citations
  4. 4Defining the Primary Immune Cell in the Immune Response to Coagulation Factor VIII 22452462026
  5. 5Factor VIII restores bone parameters and modulates muscle proteo-metabolome in Factor VIII knockout male mice2026