December 8, 2025Blood
A novel multi-target NGS application for molecular monitoring of measurable residual disease in Acute Myeloid Leukemia
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Implication
Single-center analysis shows NGS-MRD can detect MRD in AML, suggesting it aids treatment post-transplant.
Key Points
- The research aims to evaluate the effectiveness of NGS-MRD in detecting measurable residual disease in Acute Myeloid Leukemia patients.
- Single-center NGS-MRD analysis on 40 AML patients treated with allogeneic hematopoietic stem cell transplantation.
- Used novel error suppression method to analyze DNA and detect mutations relevant for MRD.
- Generated high-depth sequencing data with an extensive gene panel targeting common AML mutations.
- Identified 138 somatic mutations at diagnosis, with 57 detectable at conditioning.
- 62.5% had detectable mutations indicating MRD presence, with significant findings related to multi-mutation persistence.
- Statistical analysis indicated the persistence of two or more mutations correlates with shorter overall and relapse-free survival.
Cite This Study
Lussana et al. (2025) studied this question.