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This research aims to develop highly efficient CRISPR/Cas9 systems for controlled gene editing with reduced off-target effects.

December 12, 2025Nature CommunicationsOpen Access

Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation

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Engineered CRISPR systems enhance gene editing efficiency and minimize DNA damage, indicating potential for various cell types.

This research aims to develop highly efficient CRISPR/Cas9 systems for controlled gene editing with reduced off-target effects.
Developed conditional control methods for Cas9
Created transgenic platforms for minimizing Cas9 functionality in OFF-state
Constructed a Branaplam-regulated splice switch module
Designed a dual-control CRISPRi module for transcriptional silencing
Established systems with exceptional ON vs. OFF-state dynamic range
Minimized off-target effects in gene editing
Achieved robust Cas9 activity control across different cell types

Srinivasan et al. (2025) studied this question.

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