Single-center study shows autophagy and proteasome activity improve in patients after caloric restriction, suggesting benefits of low-calorie diets.
Key Points
This study aims to investigate the ratio of proteasome and autophagy activity in patients undergoing comprehensive wellness programs with caloric restriction.
Single-center, prospective study of 48 patients aged 18 to 64 years
Participants underwent caloric restriction (30—40% less than required) and therapeutic fasting
Protein concentrations of proteasome 20S and beclin-1 measured on day 1 and 12 using enzyme immunoassay
Body composition assessed by impedance method
Optimal proteasome 20S and autophagy ratio found in individuals with BMI of 30 kg/m2
CWP and low-calorie diet improved ratio in women and patients with BMI of 30 kg/m2, and those over 60
Therapeutic fasting periods of 3-4 and 11-12 days yielded optimal activity ratios
Lean muscle mass was a significant source of proteasome activity, while dominant components for Beclin-1 were not identified.