Experimental analysis demonstrates how mass spectrometry and light scattering evaluate polymer characteristics, suggesting methods for improving molecular‐weight distribution accuracy.
Size‐exclusion chromatography (SEC) equipped with refractive index (RI) and multi‐angle light scattering (MALS) detectors was carried out on a polystyrene (PS) sample with the weight‐average molecular weight 2.07 ×€10 4 along with the matrix‐assisted laser desorption flight time mass spectrometry (MALDI‐TOF‐MS). The MALDI‐TO‐MS result was corrected by the relative sensitivity, which was found to decay exponentially with the molecular weight. Exchanging the elution volume for molecular weight by the calibration relation, the molecular‐weight distribution (MWD) curves were obtained from the chromatograms. The MWD curve determined by the SEC chromatogram was much broader than that obtained by MALS and MALDI‐TOF‐MS because of the peak‐broadening effect. Based on the MWD determined by MALDI‐TOF‐MS, the RI chromatogram was closely described by the calculated values using the Gaussian function. Based on the results of the PS sample, RI and MALS data of polyethylene oxide samples in water were analyzed using the exponentially modified Gaussian equation.
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Kondo et al. (2025) studied this question.
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